Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 9
Endocytosis of albumin by PTECs. (a) Time response. Left panel: growth-arrested PTECs were exposed to fluorescein-labeled HSA (5 mg/ml) for 0–60 min at 37°C. Cells were then fixed with 1% paraformaldehyde and examined by fluorescence microscopy. ×1,000. Right panel: the fluorescence signals of cells incubated with FITC-HSA (5 mg/ml) for up to 4 h were counted using a fluorescence microplate reader. Results are means ± SD of triplicate experiments. (b) Dose response. The fluorescence intensity of growth-arrested PTECs exposed to FITC-HSA (1.25–20 mg/ml) for 30 min was shown. Results are means ± SD of triplicate experiments. *P = 0.001, ^†P < 0.0001, ^‡P = 0.008, ^§P = 0.036, ^#P = 0.012 versus control cells exposed to FITC-HSA at time zero (a) or to buffer alone (b).