Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 7
Role of ROS. (a) ROS induction by albumin. PTECs were exposed to HSA (1.25–20 mg/ml) for 30 min. Intracellular ROS formation was detected by the fluorescence probe, CM-H₂DCFDA, measured by flow cytometry, and expressed as percentage of MFI of control cells incubated with culture medium alone. Results are means ± SD of triplicate experiments. *P = 0.013, ^†P = 0.001, ^‡P < 0.0001 versus medium control. (b) Effect of exogenous H₂O₂. PTECs were exposed to H₂O₂ (50, 100, 200, or 400 μM) for 24 h. IL-8 protein secretion in culture supernatants was assayed by ELISA. Results are means ± SD of triplicate experiments. *P < 0.0001 versus medium control.