Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 4
FACS analysis of nuclear expression of NF-κB/Rel proteins on PTECs. (a) Confluent PTECs were incubated in serum-free medium or medium containing escalating doses of albumin as shown. After 30 min, cells were lysed, and the cell nuclei were isolated and stained with rabbit polyclonal Ab against p65 (Rel A, filled circles), or p50 (NF-κB1, open circles), or nonimmune rabbit immunoglobulins for analysis of DNA-bound NF-κB/Rel proteins by flow cytometry. Results, expressed as MFI ± SD, are obtained from triplicate experiments. *P < 0.05, ^†P = 0.006, ^‡P = 0.005, **P = 0.002, ^#P = 0.001, ^§P = 0.01 versus cells exposed to serum-free medium alone. (b) Representative flow-cytometry histograms of unstimulated and albumin-stimulated cells labeled with anti-p65 or anti-p50 subunits and FITC-conjugated secondary Ab’s.