Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 3
Polarized production of IL-8 by PTECs. Cells were grown to confluence on Transwell chambers and growth arrested. Serum-free medium alone (control) or supplemented with different concentrations of HSA was added to the apical (a) or basolateral (b) chamber, while medium alone was added to the other compartment. After overnight incubation, apical medium (white bars) and basolateral medium (black bars) were harvested for assay of IL-8 protein by ELISA. Numbers below each pair of bars represent the ratio of basolateral/apical IL-8 secretion. Results are means ± SD obtained from triplicate experiments of one kidney preparation. (a) *P = 0.002, ^†P = 0.006, ^‡P = 0.018 versus control basolateral compartment; **P = 0.038, ^##P = 0.009 versus control apical compartment; ^#P = 0.037, ^§P = 0.005 versus corresponding apical compartments. (b) *P = 0.024, ^‡P = 0.02 versus control basolateral compartment; ^†P = 0.003 versus control apical compartment; ^§P = 0.02 versus corresponding apical compartment.