Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 2
Effect of albumin from different suppliers, heat or protease pretreatment, and nonalbumin serum proteins. Confluent, growth-arrested PTECs were cultured for 24 h with 10 mg/ml of HSA from different suppliers (shown in parentheses), human transferrin, human IgG, boiled HSA (HSA boiled for 10 min), or trypsin-digested HSA (HSA digested with trypsin-conjugated agarose for 2 h). IL-8 protein secretion was measured in cell culture supernatants by ELISA. Results are means ± SD of triplicate experiments. *P < 0.0001, ^†P = 0.001 versus cells incubated in serum-free medium alone; ^#P < 0.0001 versus cells treated with HSA 1, HSA 2, or HSA 3.