Albumin stimulates interleukin-8 expression in proximal tubular epithelial cells in vitro and in vivo
J. Clin. Invest. Sydney Tang, et al. 111:515 doi:10.1172/JCI16079 [Go to this article.]

Figure 10
Effect of PI3K and PKC inhibition on albumin-induced intracellular ROS generation and IL-8 secretion in PTECs. (a) ROS generation. PTECs were exposed for 30 min to medium alone (control), HSA (10 mg/ml), LY294002 (100 μM, added 1 h before the addition of HSA), wortmannin (500 nM, added 1 h before the addition of HSA), GF109203X (5 μM, added 1 h before the addition of HSA), or staurosporin (100 nM, added 1 h before the addition of HSA). Intracellular ROS formation was detected by flow cytometry and expressed as percentage of MFI of control cells incubated with culture medium alone. Results are means ± SD of triplicate experiments. *P < 0.0001 versus medium control, ^†P = 0.001 versus cells treated with HSA alone. (b) IL-8 protein secretion. PTECs were treated for 24 h with medium alone (control), HSA (10 mg/ml), LY294002 (100 μM, added 1 h before the addition of HSA), wortmannin (500 nM, added 1 h before the addition of HSA), GF109203X (5 μM, added 1 h before the addition of HSA), or staurosporin (100 nM, added 1 h before the addition of HSA). At the end of incubation, IL-8 level was measured in supernatants using ELISA, while cell number was counted. Results are means ± SD of triplicate experiments. *P < 0.0001 versus medium control, ^†P < 0.0001 versus cells treated with HSA alone.