Spontaneous circulation of myeloid-lymphoid–initiating cells and SCID-repopulating cells in sickle cell crisis
J. Clin. Invest. Christopher E.D. Lamming, et al. 111:811 doi:10.1172/JCI15956 [Go to this article.]

Figure 7
More LTC-ICs, NK-ICs, and ML-ICs are mobilized in PB following GCSF+SCF than following GCSF-only mobilization. Single CD34⁺CD38^–Lin^– cells, selected by FACS from lymphoma patients who received GCSF+SCF mobilization (66–132 wells plated) and lymphoma patients who received GSCF-only mobilization (66–132 wells plated), were plated in the ML-IC assay as previously described (24). The frequency of LTC-ICs was enumerated by overlaying of cultures with clonogenic medium after 5 weeks. Cells were maintained for 2 weeks in expansion medium, and progeny were replated in two LTC-IC and two NK-IC cultures. The frequency of NK-ICs was enumerated by harvesting of plates after 5 weeks and evaluation of the cells for presence of CD56⁺ NK cells or CD19⁺ B cells by FACS as previously described (24). An ML-IC was identified when progeny of the initial CD34⁺CD38^–Lin^– cell gave rise to at least one LTC-IC and at least one NK-IC. Significantly more NK-ICs and ML-ICs were present in CD34⁺CD38^–Lin^– cells from blood mobilized with GCSF+SCF than in those from blood mobilized with GCSF only. Differences in LTC-IC frequency between the two groups were not significant. Differences between the groups were evaluated by t test.