Spontaneous circulation of myeloid-lymphoid–initiating cells and SCID-repopulating cells in sickle cell crisis
J. Clin. Invest. Christopher E.D. Lamming, et al. 111:811 doi:10.1172/JCI15956 [Go to this article.]

Figure 1
FACS analysis for CD34⁺CD38^–Lin^– cells in PB of NL donors and SS-SCD and AC-SCD patients. CD34⁺ cells were selected as described in Methods, stained with CD34 APC, CD38 FITC, and lineage phycoerythrin (Lin-PE) antibodies, and selected by FACS. Live cells were gated in R1, and then CD34 cells were gated against lineage cells. R2 represents the CD34⁺Lin^– gate. Cells in R2 were gated as CD34 versus CD38. The R3 gate, CD34⁺CD38^– (not shown here), was used to sort for single-cell deposition for assays.