Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 6
Addition of IgE leads to sustained FcεRI surface expression in DCs from atopic donors in a BFA- and CHX-sensitive process without affecting de novo synthesis of FcεRIα and FcεRIγ chains. (a) IgE was added from day 0 of DC culture (atopic donors) with GM-CSF and IL-4. Immunolabeling and flow-cytometric analysis of FcεRI surface expression was performed as described for Figure 1. Percentage of positive cells shown under a are the result of six independent experiments. (b) MoDCs (day 4) were incubated for 24 and 48 h with or without the addition of 1 μg/ml human myeloma IgE. After RNA isolation from highly purified MoDCs and reverse transcription, FcεRIα and FcεRIγ expression was analyzed by semiquantitative PCR using β-actin as a control. Shown is a representative experiment from an atopic donor of five total experiments. In parallel, FcεRIγ protein levels of one representative experiment of MoDC on day 6 of culture incubated with (+ IgE) and without IgE (– IgE) until day 4 of culture are shown (c). (d and e) MoDCs were generated with GM-CSF and IL-4 until day 4 of culture. Then IgE, CHX, and BFA (all 1 μg/ml) were added as indicated. Flow-cytometric analyses of FcεRI expression were done on days indicated. Mean FcεRI expression ± SEM (n = 7) is shown as percentage of FcεRI expression of monocytes at day 0.