Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 5
Significant FcεRIγ expression and association of FcεRIα with FcεRIγ can be detected only in FcεRI^pos DCs from atopic donors. (a) FcεRIγ expression was determined by double immunolabeling of MoDCs using anti-FcεRIγ and anti-CD1a after a mild permeabilization with digitonin to preserve surface-expressed FcεRI complexes. Determination of FcεRI surface expression and flow-cytometric analysis was done as described for Figure 1. (b) FcεRI^neg (4% positive cells) and FcεRI^pos (86% positive cells) DCs were subject to mild lysis with a buffer containing 1% digitonin followed by immunoprecipitation using 3G6 anti-FcεRIα Ab and SDS-PAGE. FcεRIγ coprecipitated with FcεRIα was detected by immunoblotting using 4D8 Ab. PBMC lysates were used as positive controls.