Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 4
Monocytes, FcεRI^pos, and FcεRI^neg DCs show a different localization of FcεRI subunits. Cells were adhered to coverslips, fixed, and permeabilized using 0.1% saponin. After blocking using human IgG Fc, sequential indirect immunolabeling with Ab against FcεRI subunits and organelle markers was performed. After mounting, the samples were analyzed by confocal laser scanning microscopy.