Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 3
Immature FcεRIα is found both in FcεRI^pos from atopics and FcεRI^neg DCs from nonatopics, whereas mature FcεRIα is found only in FcεRI^pos DCs. Lysates from FcεRI^neg and FcεRI^pos DCs were subject to immunoprecipitation using 3G6 anti-FcεRIα Ab. Precipitated FcεRIα was treated with Endo H, fractionated by SDS-PAGE, and then immunoblotted using RAB1 anti-FcεRIα Ab to detect immature (Endo H–sensitive) or mature (Endo H–resistant) forms. Nil, without Endo H treatment.