Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 2
DCs generated from monocytes accumulate FcεRIα, whereas FcεRIγ and Fcγ receptors are downregulated during differentiation. (a and b) Lysates from monocytes or MoDCs (> 90% purity), as well as RBL 4-48 cells stably expressing human FcεRIα, A431 epithelial cells, and PBMCs used as controls, were fractionated on SDS-PAGE and immunoblotted with Ab for FcεRIα or FcεRIγ. (c) Expression of the Fcγ receptors CD16, CD32, and CD64 was determined by flow cytometry as described for a. (d) Mature DCs were generated by addition of TNF-α to MoDC cultures. Flow cytometric analysis of FcεRI expression of six donors was done as described for Figure 1a.