Evidence for a differential expression of the FcεRIγ chain in dendritic cells of atopic and nonatopic donors
J. Clin. Invest. Natalija Novak, et al. 111:1047 doi:10.1172/JCI15932 [Go to this article.]

Figure 1
FcεRI surface and intracellular expression in monocytes and DCs from nonatopic and atopic donors. (a) For surface staining, cells were subject to double immunolabeling using anti-FcεRIα mAb 22E7, anti-CD14 or anti-CD1a mAb, and 7-aminoactinomycin-D for dead cell discrimination. For intracellular staining, fixation with 4% formaldehyde and permeabilization with 0.5% saponin was done before staining. Acquisition was performed on a FACSCalibur flow cytometer. Shown are histograms from CD14^pos or CD1a^pos cells (day 8), which represent typical data for at least ten experiments for each donor group. (b) Data were obtained as described for (a). Shown are at least three experiments for each parameter (mean of percentage of positive cells ± SEM).