Allergen-specific CD8+ T cells and atopic disease
J. Clin. Invest. Suranjith L. Seneviratne, et al. 110:1283 doi:10.1172/JCI15753 [
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Figure 1(
a–
c) Illustration of the number of CD8
+ T cells staining with each tetramer (P3 A, P5 B, P6 C) per million CD8
+ T cells for each of the subject groups. NA, nonatopic; AA, atopic asymptomatic; S, atopic symptomatic. (
d) An example of tetramer staining of PBMCs from an A*0201–positive atopic symptomatic donor with the P6-based tetramer:
x axis represents staining with tetramer and
y axis represents staining with anti-CD8. In this example, 0.12% of CD8
+ T cells stained with the tetramer. (
e and
f) Examples of negative control staining with
e showing PBMCs from an A*0201–positive nonatopic donor and
f showing PBMCs from an A*0201–positive atopic stained with an A*0201-tetramer containing a peptide derived from hepatitis B core. The horizontal gates have been applied to include the CD8-high population.
