Felix Kreier, Eric Fliers, Peter J. Voshol, Corbert G. Van Eden, Louis M. Havekes, Andries Kalsbeek, Caroline L. Van Heijningen, Arja A. Sluiter, Thomas C. Mettenleiter, Johannes A. Romijn, Hans P. Sauerwein, Ruud M. Buijs
Hormone mRNA expression in adipose tissue after parasympathetic denervation. The left retroperitoneal fat pad was parasympathetically denervated (n = 9) and compared with the right intact pad for the expression of mRNA of resistin, leptin, adiponectin, and elongation factor–1α (as a reference gene) by means of real-time RT-PCR. Sham-operated animals were used as control (n = 5). While resistin and leptin mRNA expression was reduced (–71%, Mann-Whitney U test, *P = 0.001; –45%, Mann-Whitney U test, **P = 0.004, respectively), adiponectin and reference mRNA did not change significantly. Thus, parasympathetic denervation of adipose tissue specifically changes mRNA expression of fat-derived hormones. One relative unit is the equivalent cDNA corresponding with 0.1 μg per well of the pooled cDNA of the control fat pads. Values are expressed as mean ± SEM.