Felix Kreier, Eric Fliers, Peter J. Voshol, Corbert G. Van Eden, Louis M. Havekes, Andries Kalsbeek, Caroline L. Van Heijningen, Arja A. Sluiter, Thomas C. Mettenleiter, Johannes A. Romijn, Hans P. Sauerwein, Ruud M. Buijs
Uptake of glucose and FFA, and HSL activity in adipose tissue after parasympathetic denervation. The left retroperitoneal fat pad was either parasympathetically denervated (n = 6) or sham operated (n = 6). Using a hyperinsulinemic euglycemic clamp, the uptake of 3H-2-deoxy-D-glucose and 14C-palmitate and the activity of the catabolic enzyme HSL were defined. Under these hyperinsulinemic conditions, glucose uptake in the denervated fat pad was reduced by 33% (by Mann-Whitney U test, *P = 0.02) and FFA by 36% (Mann-Whitney U test, *P = 0.02); HSL activity increased by 51% (Mann-Whitney U test, **P = 0.03). Thus, parasympathetic denervation of adipose tissue shifts the metabolism to a catabolic state: uptake of substrate is decreased, while lipolysis increases. Values are expressed as mean ± SEM. dpm, disintegrations per minute.