Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair
J. Clin. Invest. Xinping Zhang, et al. 109:1405 doi:10.1172/JCI15681 [
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Figure 3Quantitative analysis of fracture callus in wild-type and
COX-2–/– mice at 7, 14, and 21 days after fracture. Tissue sections from wild-type and
COX-2–/– mice harvested on days 7 (
n = 5), 14 (
n = 7), and 21 (
n = 9) after fracture were stained with Alcian blue/hematoxylin or for tartrate resistant acid phosphate (TRAP) as described in Methods. All slides were analyzed using Osteometrics software, (Osteometrics Inc., Atlanta, Georgia, USA) and percentages of mesenchymal tissue (
a), bone (
b), and cartilage (
c) relative to the total fracture area are presented as the mean ± SEM. (
d) Osteoclast numbers (TRAP-positive cells) per mm
2 bone area were quantified as described in Methods and are presented as the mean ± SEM. Student’s
t test was used to determine the statistical differences between each group. *
P < 0.05, ***
P < 0.001.
