Novel synthetic amino acid copolymers that inhibit autoantigen-specific T cell responses and suppress experimental autoimmune encephalomyelitis
J. Clin. Invest. Masha Fridkis-Hareli, et al. 109:1635 doi:10.1172/JCI15402 [Go to this article.]

Figure 2
Inhibition of HLA-DR2–restricted MBP 84-102–specific T cells 2E12, 8073, and Hy1B in the presence of the random copolymers. Irradiated MGAR cells were co-incubated in duplicate with the MBP 85-99 at the final concentration of 12.5 μM and different concentrations of the random copolymers for 2 hours at 37°C; then T cells were added and incubated for 24 hours at 37°C. Supernatants (30 μl) were incubated with IL-2–dependent cytotoxic T lymphoma line cells, followed by labeling with ³H-thymidine (1 μCi/well) for 12 hours. Other details were as described in Methods.