The Fas-associated death domain protein suppresses activation of NF-κB by LPS and IL-1β
J. Clin. Invest. Douglas D. Bannerman, et al. 109:419 doi:10.1172/JCI14774 [
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Figure 4Deletion of FADD enhances LPS- and IL-1β–induced degradation of IκB.
FADD+/+ and
FADD–/– MEFs were incubated with medium, LPS (100 ng/ml), or IL-1β (10 ng/ml) for increasing exposure times, and lysates derived from these cells were immunoblotted with antibodies raised against either IκB-α or IκB-β (
a and
b). In other experiments,
FADD+/+ MEFs stably expressing GFP (+/+ GFP) or
FADD–/– MEFs stably expressing either GFP (–/– GFP) or FADD (–/– + FADD) were treated with LPS (100 ng/ml) or IL-1β (10 ng/ml) for 45 minutes, and lysates were immunoblotted as above (
c).
