The Fas-associated death domain protein suppresses activation of NF-κB by LPS and IL-1β
J. Clin. Invest. Douglas D. Bannerman, et al. 109:419 doi:10.1172/JCI14774 [
Go to this article.]
Figure 3Reconstitution of FADD reverses the enhanced NF-κB activation and NF-κB–dependent gene expression in
FADD–/– MEFs exposed to LPS or IL-1β. Lysates derived from
FADD–/– MEFs stably transfected with either GFP vector alone or FADD were immunoblotted with anti-human FADD antibody to confirm expression (
a). Molecular mass (in kDa) is indicated. In other experiments,
FADD–/– MEFs expressing either GFP or FADD were treated for 4.5 hours with medium, LPS (100 ng/ml), or mIL-1β (10 ng/ml), lysed, and assayed for luciferase activity (
b). Alternatively, MEFs were treated for 12 hours and the culture supernatants analyzed for IL-6 (
c) or KC (
d). Vertical bars represent mean (± SE) luciferase activity in arbitrary units (
b) or pg/ml (
c and
d). *Significantly decreased compared with GFP-expressing cells exposed to the same treatment.
