The Fas-associated death domain protein suppresses activation of NF-κB by LPS and IL-1β
J. Clin. Invest. Douglas D. Bannerman, et al. 109:419 doi:10.1172/JCI14774 [Go to this article.]

Figure 2
Deletion of FADD enhances LPS- and IL-1β–induced NF-κB activity and NF-κB–dependent gene expression. FADD^+/+ and FADD^–/– MEF lysates were immunoblotted with anti-murine FADD antibody to confirm the genetic phenotype of these cells (a). Molecular mass (in kDa) is indicated. In other experiments, FADD^+/+ and FADD^–/– MEFs were treated for 4.5 hours with medium, LPS (100 ng/ml), or mIL-1β (10 ng/ml), lysed, and assayed for luciferase activity (b). Alternatively, MEFs were treated for 12 hours and the culture supernatants analyzed for IL-6 (c) or KC (d). Vertical bars represent mean (± SE) luciferase activity in arbitrary units (b) or pg/ml (c and d). *Significantly increased compared with FADD^+/+ MEFs exposed to the same treatment.