The Fas-associated death domain protein suppresses activation of NF-κB by LPS and IL-1β
J. Clin. Invest. Douglas D. Bannerman, et al. 109:419 doi:10.1172/JCI14774 [Go to this article.]

Figure 1
Overexpression of FADD or the DD of FADD inhibits LPS- and IL-1β–induced NF-κB activation. HMEC-1 cells were stably transfected with GFP vector alone (a–d), the DD of FADD (a and b), or full-length FADD (c and d), and expression was confirmed by Western blot analysis (a and c). In separate studies, these cells were treated for 4.5 hours with medium, LPS (100 ng/ml), or hIL-1β (100 ng/ml), lysed, and assayed for luciferase activity (b and d). Vertical bars represent mean (± SE) luciferase activity in arbitrary units. *Significantly decreased compared with HMEC-1 cells transfected with vector alone that were exposed to identical treatment.