Hypothalamic Y2 receptors regulate bone formation
J. Clin. Invest. Paul A. Baldock, et al. 109:915 doi:10.1172/JCI14588 [Go to this article.]

Figure 5
Expression of GFP and in situ PCR of the hypothalamus of Cre-adenovirus–injected Y2^lox/lox mice and germline Y2^–/– mice. (a) Fluorescence micrograph of the hypothalamus of a Y2^lox/lox animal 21 days after injection of GFP-expressing adenovirus. Bar represents 2 mm. (b) Schematic drawing of the position of the primers (oligo C, D, and E) used for in situ PCR. (c) In situ PCR on a brain section from a Y2^lox/lox animal using oligo C and oligo D. (d) In situ PCR on a brain section from a Y2^–/– animal using oligo C and oligo D. (e) In situ PCR on a brain section from a Cre-adenovirus–injected Y2^lox/lox animal using oligo C and oligo E. (f) In situ PCR on a brain section from a Cre-adenovirus–injected Y2^lox/lox animal using oligo C and oligo E without enzyme. Bar represents 1 mm in c–f.