Targeted disruption of the Chop gene delays endoplasmic reticulum stress–mediated diabetes
J. Clin. Invest. Seiichi Oyadomari, et al. 109:525 doi:10.1172/JCI14550 [Go to this article.]

Figure 5
Immunohistochemical detection of Chop and apoptosis in islets of male Ins2^WT/WTChop^+/+, Ins2^WT/C96YChop^+/+, and Ins2^WT/C96YChop^–/– mice. (a) Immunostaining of insulin and Chop. The pancreata from Ins2^WT/C96YChop^+/+ and Ins2^WT/C96YChop^–/– male mice at 4 weeks of age were fixed and then costained for insulin (red) and Chop (green). Phase-contrast images and fluorescence images of the same fields are shown. Original magnification: ×100. (b) Apoptosis detection by the TUNEL method. The pancreata from Ins2^WT/WTChop^+/+, Ins2^WT/C96YChop^+/+, and Ins2^WT/C96YChop^–/– male mice were obtained and costained for insulin (red) and TUNEL-positive cells (green). Phase-contrast images and fluorescence images of the same fields are shown. Original magnification: ×200.