Targeted disruption of the Chop gene delays endoplasmic reticulum stress–mediated diabetes
J. Clin. Invest. Seiichi Oyadomari, et al. 109:525 doi:10.1172/JCI14550 [Go to this article.]

Figure 3
Apoptosis in MIN6 cells overexpressing Ins2^C96Y–EGFP and Ins2^C96Y. (a) Cells were transfected with pIns2^WT-EGFP or pIns2^C96Y-EGFP. At the indicated times after transfection, cells were observed under a fluorescence microscope. Original magnification: ×100. (b) Cells were cotransfected with pEYFP-ER and either pcDNA-Ins2^WT or pcDNA-Ins2^C96Y. Forty-eight hours after transfection, cells were observed under a fluorescence microscope. Original magnification: ×200. (c) Cells were cotransfected with pEGFP and either pcDNA-Ins2^WT or pcDNA-Ins2^C96Y. The transfected cells and apoptotic cells were visualized by GFP fluorescence and Hoechst 33258 staining, respectively. pcDNA-Ins2^WT–transfected cells were not apoptotic (arrowheads), whereas pcDNA-Ins2^C96Y–transfected cells were apoptotic (arrows). Original magnification: ×400. (d) Cells were transfected with pcDNA-Ins2^WT or pcDNA-Ins2^C96Y. DNA was extracted, electrophoresed in 2% agarose gel, stained with SYBR Green I, and visualized by UV transillumination. (e) Cells were cotransfected with pEGFP and either pcDNA-Ins2^WT or pcDNA-Ins2^C96Y. The transfected cells and apoptotic cells were visualized by GFP fluorescence and annexin V staining, respectively. Original magnification: ×200.