Autologous dendritic cells transfected with prostate-specific antigen RNA stimulate CTL responses against metastatic prostate tumors
J. Clin. Invest. Axel Heiser, et al. 109:409 doi:10.1172/JCI14364 [Go to this article.]

Figure 2
In vivo induction of PSA-specific T cell responses. PBMCs obtained at baseline (PSA, KK pre) and after three vaccination cycles (PSA, KK post) using PSA RNA–transfected DCs were cultured overnight with PSA or kallikrein protein (5 μg/ml). The specific T cell frequencies in each of the evaluable patients treated at a low dose level (10⁷ cells; patient 2, 3, and 5), medium dose level (3 × 10⁷ cells; patient 6, 7, and 9), or high dose level (5 × 10⁷ cells; patient 11 and 13) is expressed as the number of spot-forming cells per 5 × 10⁵ PBMCs seeded in each well.