Autologous dendritic cells transfected with prostate-specific antigen RNA stimulate CTL responses against metastatic prostate tumors
J. Clin. Invest. Axel Heiser, et al. 109:409 doi:10.1172/JCI14364 [Go to this article.]

Figure 1
Incubation of immature DCs with PSA RNA provides a maturation stimulus. (a) Upregulation of CD83 expression by immature DCs in the presence of increasing PSA mRNA concentrations. (b–e) Immature DCs were incubated for 45 minutes in the presence of 20 μg/ml of PSA mRNA and analyzed after 48 hours by flow cytometry for expression of the DC-specific marker CD1a and the DC maturation marker CD83, respectively. (b) Staining with isotype immunoglobulins IgG_2b-FITC and IgG₁-PE (isotype control). (c) DCs incubated in the absence of PSA mRNA (untreated). (d) DCs incubated with PSA mRNA that had been treated for 1 hour at 37°C with 10 μg/ml RNase A (PSA-RNA + RNase A), and (e) DCs incubated in the presence of PSA mRNA. One representative experiment out of three independent experiments is presented.