Origin of endothelial progenitors in human postnatal bone marrow
J. Clin. Invest. Morayma Reyes, et al. 109:337 doi:10.1172/JCI14327 [Go to this article.]

Figure 2
Immunohistochemical evaluation of MAPC-derived endothelial cells. (a) MAPCs (after 65 population doublings; donor age, 22 years) were replated at 2 × 10⁴ cells/cm² in fibronectin-coated wells in serum-free defined medium with 10 ng/ml VEGF. After 14 days, cells were fixed with paraformaldehyde, permeabilized with Triton X-100, and stained with Ab’s against α_vβ₅ (scale bar = 50 μm), ZO-1, β-catenin, and γ-catenin. Cells were then evaluated by confocal fluorescence microscopy. Typical membrane staining is seen for the adhesion, α_vβ₅, and for the adhesion junction proteins ZO-1, β-catenin, and γ-catenin. Representative example from one of three total donors. Scale bar = 50 μm. (b) Morphology of MAPCs at day 0 (upper panel) and day 21 (lower panel) after VEGF treatment in bright-field microscopy. Scale bar = 25 μm.