Sophie Lanone, Tao Zheng, Zhou Zhu, Wei Liu, Chun Geun Lee, Bing Ma, Qingsheng Chen, Robert J. Homer, Jingming Wang, Lesley A. Rabach, Morgan E. Rabach, J. Michael Shipley, Steven D. Shapiro, Robert M. Senior, Jack A. Elias
Localization of MMP-2 and MMP-14 mRNA. (a–f) ISH was used to localize the MMP-2 mRNA in lungs from dox-treated CC10-rtTA-IL-13 transgene– and transgene+ mice. (a) A transgene– mouse incubated with antisense probe. (b) A transgene– mouse incubated with sense probe. (c) A transgene+ mouse incubated with antisense probe. (d) A transgene+ mouse incubated with sense probe. (e) A transgene+ mouse incubated with antisense probe. The large and small arrows in e illustrate inflammatory cell and epithelial cell staining, respectively. (f) A transgene+ mouse incubated with antisense probe. The large and small arrows in f illustrate negatively staining macrophages and positively staining alveolar epithelial cells, respectively. (g–k) ISH was used to localize the MMP-14 mRNA in lungs from dox-treated CC10-rtTA-IL-13 transgene– and transgene+ mice. (g) A transgene– mouse incubated with antisense probe. (h) A transgene– mouse incubated with sense probe. (i) A transgene+ mouse incubated with antisense probe. (j) A transgene+ mouse incubated with sense probe. (k) A transgene+ mouse incubated with antisense probe. The large and small arrows in k highlight positively staining alveolar epithelial cells and macrophages, respectively.