Renal chemokine receptor expression after UUO. Real-time RT-PCR was performed using total renal RNA of obstructed (UUO) and contralateral (CLK) kidneys of four to six mice 10 days after ureter ligation. Levels of mRNA expression for CCR1, CCR2, and CCR5 are expressed in relation to renal GAPDH mRNA expression as indicated in Methods. The expression in UUO kidneys of control mice is set as 1. (a) A marked increase of mRNA for all three receptors was noted in UUO kidneys compared with CLK of vehicle controls. (b) Treatment with BX471 for 10 days resulted in a significant decrease of CCR1 and CCR5 mRNA expression (P = 0.005 and P = 0.03, respectively) compared with UUO kidneys of vehicle controls. For CCR2 the difference was not significant (P = 0.07). *P < 0.05.