Enhanced ERK-1/2 activation in mice susceptible to coxsackievirus-induced myocarditis
J. Clin. Invest. Mary Anne Opavsky, et al. 109:1561 doi:10.1172/JCI13971 [Go to this article.]

Figure 1
CVB3 infection activates the ERK-1/2 pathway in Jurkat cells. (a) Jurkat cells (10⁷) were lysed before (pre) or after treatment with CVB3 (moi = 50; +) or control media (–) for the indicated times. One result represents five experiments. (b) Jurkat cells (10⁶) were lysed 30 minutes to 4 hours after infection with CVB3 (moi = 50; +) or control media (–) for the indicated times. The increase in ERK-1/2 phosphorylation evident at 30 minutes to 2 hours after infection did not extend to the 4-hour time point. One result represents two experiments. (c) Five or 10 minutes after treatment with CVB3-CG (CG) or CVB3-VR30 (Nancy; N) (moi = 50), or control media (–), cells (10⁷) were lysed. One result represents three experiments. All cell lysates were immunoblotted with anti–phospho-ERK-1/2 (P-ERK-1/2) and anti–total ERK-1/2 Ab’s. Fold change in P-ERK-1/2-total ERK-1/2 ratio is indicated.