Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients
J. Clin. Invest. Dieter Worlitzsch, et al. 109:317 doi:10.1172/JCI13870 [
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Figure 3Localization of
P. aeruginosa and beads in transported and stationary ASL (mucus) produced by planar CF cultures. Representative confocal images of ASL (red) fluorescent
P. aeruginosa (green) or green fluorescent beads.
P. aeruginosa or beads were added to the air-liquid interface in 25-nl aliquots by a microsyringe mounted in a hydraulic micromanipulator. (
a) X-Z confocal image of
P. aeruginosa 3 minutes after addition to the surface of ASL (mucus) exhibiting rotational transport. (
b) X-Z confocal image of beads 3 minutes after addition to the surface of mucus exhibiting rotational transport. Note that due to the rapid “tumbling” movement of the mucus it was not possible to obtain early time-point images of
P. aeruginosa or beads at the air-liquid interface.
P. aeruginosa 3 minutes (
c) and 15 minutes (
d) after addition to stationary mucus. Beads at 3 minutes (
e) and 15 minutes later (
f) after addition to stationary mucus. Scale bars, 100 μm.
