Pulmonary overexpression of IL-9 induces Th2 cytokine expression, leading to immune pathology
J. Clin. Invest. Ulla-Angela Temann, et al. 109:29 doi:10.1172/JCI13696 [Go to this article.]

Figure 8
Histologic staining for mucin after cytokine blockade. Light micrographs of lung sections stained with AB/PAS from IL-9 transgene-negative (a) or –positive (b–f) mice that were housed for 14 days with doxycycline-supplemented food. During the entire time of IL-9 transgene induction selected groups of animals received Ab’s: (c) control Ab’s, hIgG; (d) anti–IL-4; (e) sIL-13Rα-Fc; (f) anti–IL-4 and sIL-13Rα-Fc. Lung sections from transgene-positive mice that received anti–IL-4 Ab’s, control Ab’s, or no Ab’s showed strong positive (magenta) staining for mucin in airway epithelial cells (arrows) and accumulation of inflammatory cells near blood vessels and airways (b–d). Infiltration of lung tissue with inflammatory cells or positive staining for mucin was not observed in sections from transgene-positive mice that received sIL-13Rα-Fc alone (e), or in combination with anti–IL-4 (f), or in transgene-negative mice (a). Original magnification ×300.