Pulmonary overexpression of IL-9 induces Th2 cytokine expression, leading to immune pathology
J. Clin. Invest. Ulla-Angela Temann, et al. 109:29 doi:10.1172/JCI13696 [Go to this article.]

Figure 7
Characterization of inflammatory cells in lung lavage fluid after cytokine blockade. IL-9 transgene-negative (TG^–) or -positive (TG⁺) mice were housed with (+Dox) or without (–Dox) doxycycline-supplemented food for 14 days. During the entire time of IL-9 transgene induction, selected groups of animals received anti–IL-4 Ab’s, sIL-13Rα-Fc, or isotype control Ab’s (hIgG) as described in Methods. At day 14, mice were sacrificed and the lung lavaged. (a) Total lung lavage cell counts. (b) Differential cell counts on lung lavage cells. Results are expressed as total cell numbers and were obtained from at least 200 cell counts. Data are expressed as mean ± SD. Significant differences in total cell number (a) or number of eosinophils (b) compared with that of transgene-positive mice that received doxycycline but no Ab’s are indicated with an asterisk.