Upregulation of TRAF-3 by shear stress blocks CD40-mediated endothelial activation
J. Clin. Invest. Carmen Urbich, et al. 108:1451 doi:10.1172/JCI13620 [
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Figure 4CD40L-induced MCP-1 expression is mediated by AP-1. (
a and
c) HUVECs were preincubated for 30 minutes with PDTC, curcumin, or NDGA, then stimulated for 6 hours with CD40L. MCP-1 mRNA was detected by Northern blot analysis. 28S RNA served as loading control. Blots representative of three experiments are shown. (
b) HUVECs were transfected with AP-1 decoy or scrambled oligonucleotides and stimulated with CD40L for 6 hours. MCP-1 mRNA was detected by Northern blot analysis. 28S RNA served as loading control. A representative blot from five experiments is shown. (
d) HUVECs were incubated with CD40L in the presence or absence of shear stress or TNF-α (as positive control). IκB-α protein was analyzed by Western blotting, with actin as loading control.
