Upregulation of TRAF-3 by shear stress blocks CD40-mediated endothelial activation
J. Clin. Invest. Carmen Urbich, et al. 108:1451 doi:10.1172/JCI13620 [
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Figure 3Role of TRAF-3 in CD40 signaling. (
a–
c) Effect of TRAF-3 overexpression on CD40L-stimulated MCP-1 expression (
a, right), IL-8 release (
b), and tissue factor activity (
c). HUVECs were transfected with TRAF-3 for 18 hours before stimulation of cells with CD40L for 6 hours. Expression of TRAF-3 is shown in
a (left). Data are mean ± SEM. (
d) HUVECs were transfected with a dominant-negative TRAF-3 construct (dnTRAF3; amino acids 340–568) and preexposed to shear stress for 12 hours before stimulation with CD40L for an additional 6 hours under continuous application of shear stress. MCP-1 mRNA expression was detected by Northern blot. The expression of dnTRAF3 is shown in the left panel. MCP-1 expression in response to shear stress and/or CD40L is shown in the right panel. HA, hemagglutinin.
