Upregulation of TRAF-3 by shear stress blocks CD40-mediated endothelial activation
J. Clin. Invest. Carmen Urbich, et al. 108:1451 doi:10.1172/JCI13620 [Go to this article.]

Figure 1
Shear stress prevents CD40L-induced endothelial cell activation. HUVECs were preexposed to shear stress for 12 hours before stimulation with recombinant CD40L for 6 hours under continuous flow conditions. MCP-1 mRNA expression was monitored by Northern blot analysis as representatively shown (a). The quantification is shown in (b); data are mean ± SEM. n = 4, *P < 0.05 vs. CD40L stimulation. (c) Shear stress inhibits CD40-induced tissue factor activity. After preexposure of HUVECs to shear stress, tissue factor activity was detected using a clotting assay. Data are mean ± SEM. n = 4, *P < 0.05 vs. CD40L stimulation. (d) Platelets were activated as described in Methods and incubated with HUVECs that had been preexposed to shear stress for 12 hours. MCP-1 expression was detected after 6 hours of coincubation. A representative Northern blot is shown. Quantification was performed with four experiments. P < 0.05 vs. activated platelets. TF, tissue factor.