Dipeptidyl peptidase I activates neutrophil-derived serine proteases and regulates the development of acute experimental arthritis
J. Clin. Invest. April M. Adkison, et al. 109:363 doi:10.1172/JCI13462 [
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Figure 2In vitro PMN function and in vivo response to thioglycollate in the absence of DPPI. (
a) Bone marrow–derived PMNs from WT and
DPPI–/– mice were allowed to migrate across a filter in response to 10
–4 M fMLP, 2% zymosan-activated rat serum (ZAS), or 3 μg/ml rhIL-8. The number of cells that migrated was expressed as a percentage of total PMNs. Values represent the mean ± SEM of at least three animals. (
b) Superoxide production by PMNs in response to PMA, expressed as nmol of O
2– generated per 10
6 PMNs (
n > 3 per genotype). The number of PMNs and macrophages recruited in response to intraperitoneal injection of thioglycollate is normal in
DPPI–/– mice at 4 hours (
c) 24 hours (
d) and 120 hours (
e).The slightly lower number of macrophages at 120 hours after thioglycollate injection in the
DPPI–/– mice did not reach statistical significance (
n = 4–5 mice per genotype). Mph, macrophages.
