MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If
J. Clin. Invest. Barbara Schenk, et al. 108:1687 doi:10.1172/JCI13419 [Go to this article.]

Figure 3
Analysis of total Dol-P levels in a healthy individual (control subject) and patient L. Dol-P was extracted from approximately 10⁹ EBV-transfected lymphoblasts grown in liquid culture and analyzed. Isoprenologues were separated by HPLC and detected using an ultraviolet detector at 214 nm. Dol-P composed of 18 isoprene units (Dol-18-P) was added to the cells before extraction and served as a qualitative and quantitative marker. Dol-Ps isolated from genetically tailored yeast cells served as standards for the Dol-Ps composed of 15–21 isoprene units (Dol-P standards). The chain length of the isoprenologues is indicated above the peaks.