JCI - Reduced expression of the murine p85α subunit of phosphoinositide 3-kinase improves insulin signaling and ameliorates diabetes

Reduced expression of the murine p85α subunit of phosphoinositide 3-kinase improves insulin signaling and ameliorates diabetes

Franck Mauvais-Jarvis, Kohjiro Ueki, David A. Fruman, Michael F. Hirshman, Kei Sakamoto, Laurie J. Goodyear, Matteo Iannacone, Domenico Accili, Lewis C. Cantley, C. Ronald Kahn

Published in Volume 109, Issue 1

J Clin Invest. 2002; 109(1):141–149 doi:10.1172/JCI13305

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Figure 1

Creation of mutant mice. (a) Mice were bred and genotyped using PCR. The presence of the IR mutant allele results in a 240-bp product, while there is no product in the wild-type (WT) allele. The presence of the wild-type allele for the IRS-1 gene results in a 440-bp product; insertion of the neomycin cassette in the targeted allele results in a band of 1.25 kb. In the case of the Pik3r1 gene, the mutant allele results in a band at 600 bp, whereas the wild-type allele yields a 750-bp product. (b) Body weight of the various subgroups of mice was determined at the indicated ages. Results are expressed as mean ± SEM (n = 12–30 mice per genotype).