Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes
J. Clin. Invest. N. Manjunath, et al. 108:871 doi:10.1172/JCI13296 [Go to this article.]

Figure 5
Function of activated CD8⁺ T cells differentiated with IL-2 and IL-15 after adoptive transfer. P14 Tg splenocytes were stimulated with peptide for 2 days, washed, and cultured with a high (20 ng/ml; AT/IL-2 Hi) or low (5 ng/ml; AT/IL-2 Lo) dose of IL-2 or IL-15 (20 ng/ml; AT/IL-15) for 5 more days and infused intravenously into groups of C57/BL6 mice (10⁶ cells per mouse; n = 3 mice per group). Mice were also infused with naive CD8⁺ T cells from P14 Tg mice (AT/Naive) or with PBS (No AT) as controls. To generate memory cells in vivo, some AT/Naive mice were infected with recombinant vaccinia virus encoding LCMV glycoprotein 2 days after transfer (AT/Naive/rVV). All mice were rested for 10 weeks, after which they were challenged with recombinant Listeria monocytogenes encoding the gp33 epitope; on day 4 after infection, PELs were tested for peptide-specific IFN-γ production (a) and cytotoxicity (b). Mean ± SD of all three experiments are shown in a, and results from one representative experiment (of three performed with similar results) are shown in b.