Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes
J. Clin. Invest. N. Manjunath, et al. 108:871 doi:10.1172/JCI13296 [
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Figure 2IL-2 and IL-15 induce distinct phenotypic changes in activated CD8
+ T cells. Splenocytes from P14XT-GFP double-Tg mice were either examined directly (naive cells) (
a), or stimulated with gp33 peptide and examined after 2 days (peptide D2) (
b), or stimulated with peptide for 2 days and then cultured in the presence of 20 ng/ml of either IL-2 (+ IL-2) (
c), or IL-15 (+IL-15) (
d) for the next 5 days and analyzed by flow cytometry for size, GFP expression, and expression of surface markers. Representative results from one experiment (of three performed) are shown after gating on CD8
+ T cells. Quadrant statistics are shown as percentage of gated cells. Low concentrations of IL-2 (5 ng/ml or less) induced phenotypic changes in primed CD8 T cells similar to IL-15 (not shown). P-sel, P-selectin.
