Immunoglobulin heavy chain expression shapes the B cell receptor repertoire in human B cell development
J. Clin. Invest. Eric Meffre, et al. 108:879 doi:10.1172/JCI13051 [Go to this article.]

Figure 1
Immunoglobulin rearrangements in human pro-B cells. (a) B cell precursors in sibling control (left), Igμ^–/– (middle), and Igμ^Δ (right) bone marrow. CD34⁺CD19⁺ pro-B cells from control and both Igμ-deficient patients were sorted as gated. (b) Heavy and light chain Ig gene expression in human pro-B cells. RNA from FACS-sorted CD34⁺CD19⁺ pro-B cells from both Igμ-deficient patients and control was analyzed by semiquantitative RT-PCR using 5′ consensus V_H, V_κ, or V_λ and 3′ C_μ, C_κ, or C_λ primers, respectively, and visualized by ³²PdATP incorporation. “Neg.” denotes a negative control without cDNA for RT-PCR reactions. Actin RT-PCR was used as mRNA loading control. Serial fivefold dilutions of cDNA are shown.