Article tools
Author information
Published in Volume 101, Issue 7 (April 1,1998)
J. Clin. Invest. 101(7): 1296-1300 (1998). doi:10.1172/JCI1259.
Copyright © 1998, The American Society for Clinical Investigation.

Research Article

Increased expression of the sodium/iodide symporter in papillary thyroid carcinomas.

T Saito, T Endo, A Kawaguchi, M Ikeda, R Katoh, A Kawaoi, A Muramatsu and T Onaya

Third Department of Internal Medicine, Yamanashi Medical University, Tamaho, Yamanashi 409-38, Japan.

Published April 1, 1998

Iodide is concentrated to a much lesser extent by papillary thyroid carcinoma as compared with the normal gland. The Na+/I- symporter (NIS) is primarily responsible for the uptake of iodide into thyroid cells. Our objective was to compare NIS mRNA and protein expression in papillary carcinomas with those in specimens with normal thyroid. Northern blot analysis revealed a 2.8-fold increase in the level of NIS mRNA in specimens with papillary carcinoma versus specimens with normal thyroid. Immunoblot analysis using anti-human NIS antibody that was produced with a glutathione S-transferase fusion protein containing NIS protein (amino acids 466-522) showed the NIS protein at 77 kD. The NIS protein level was elevated in 7 of 17 cases of papillary carcinoma but was not elevated in the normal thyroid. Immunohistochemical staining revealed abundant NIS in 8 of 12 carcinomas, whereas NIS protein was barely detected in specimens with normal thyroid. Although considerable patient-to-patient variation was observed, our results indicate that NIS mRNA is elevated, and its protein tends to be more abundant, in a subset of papillary thyroid carcinomas than in normal thyroid tissue.

This article Copyright © 1998, The American Society for Clinical Investigation.

Articles that cite
this article:

The sodium iodide symporter: its pathophysiological and therapeutic implications
Christine Spitzweg, John C. Morris
Clin Endocrinol (Oxf) 57(5):559. [CrossRef]

Identification of antigenic domains on the human sodium-iodide symporter which are recognized by autoantibodies from patients with autoimmune thyroid disease
E. H. Kemp, E. A. Waterman, R. A. Ajjan, K. A. Smith, P. F. Watson, M. E. Ludgate, A. P. Weetman
Clin Exp Immunol 124(3):377. [CrossRef]

Molecular and cellular mechanisms of transepithelial iodide transport in the thyroid
Mikael Nilsson
10(2-3):277. [CrossRef]

Distribution of Na+/I– Symporter in Thyroid Cancers in an Iodine-deficient Population: An Immunohistochemical Study
Anjali Mishra, Lily Pal, Saroj Kanta Mishra
World J Surg 31(9):1737. [CrossRef]

Functional Characterization of Human Thyroid Tissue with Immunohistochemistry
Antongiulio Faggiano, Bernard Caillou, Ludovic Lacroix, Monique Talbot, Sebastiano Filetti, Jean-michel Bidart, Martin Schlumberger
17(3):203. [CrossRef]

Absence of Sodium/Iodide Symporter Gene Mutations in Differentiated Human Thyroid Carcinomas
Diego Russo, Diana Manole, Franco Arturi, Horacio G. Suarez, Martin Schlumberger, Sebastiano Filetti, Michael Derwahl
11(1):37. [CrossRef]

Sodium Iodide Symporter-Based Strategies for Diagnosis and Treatment of Thyroidal and Nonthyroidal Malignancies
Armin E. Heufelder, Nils Morgenthaler, Meike L. Schipper, Werner Joba
11(9):839. [CrossRef]

Lack of Correlation for Sodium Iodide Symporter mRNA and Protein Expression and Analysis of Sodium Iodide Symporter Promoter Methylation in Benign Cold Thyroid Nodules
Susanne Neumann, Katrin Schuchardt, Andreas Reske, Alexander Reske, Peter Emmrich, Ralf Paschke
14(2):99. [CrossRef]

The sodium iodide symporter and thyroid disease
Peter P. A. Smyth, Roisin M. Dwyer
Clin Endocrinol (Oxf) 56(4):427. [CrossRef]

Successful thyroid tissue ablation as defined by a negative whole-body scan or an undetectable thyroglobulin: a comparative study
Maroun Karam, Paul J. Feustel, Eric S. Postal, Ayesha Cheema, C. Richard goldfarb
Nucl Med Commun 26(4):331. [CrossRef]

Expression of the sodium iodide symporter in human kidney
Christine Spitzweg, Charyl M Dutton, Maria R Castro, Elizabeth R Bergert, John R Goellner, Armin E Heufelder, John C Morris
Kidney Int 59(3):1013. [CrossRef]

Novel germline variants identified in the inner mitochondrial membrane transporter TIMM44 and their role in predisposition to oncocytic thyroid carcinomas
E Bonora, C Evangelisti, F Bonichon, G Tallini, G Romeo
Br J Cancer 95(11):1529. [CrossRef]

Defects in iodide metabolism in thyroid cancer and implications for the follow-up and treatment of patients
Martin Schlumberger, Ludovic Lacroix, Diego Russo, Sebastiano Filetti, Jean-Michel Bidart
Nat Clin Pract Endocrinol Metab 3(3):260. [CrossRef]

MIDGE/hNIS vaccination generates antigen-associated CD8+IFN-γ+ T cells and enhances protective antitumor immunity
Yun Choi, Yong-Hyun Jeon, Joo-Hyun Kang, June-Key Chung, Manuel Schmidt, and Chul-Woo Kim
Int J Cancer 120(9):1942. [CrossRef]

PTTG and PBF repress the human sodium iodide symporter
K Boelaert, V E Smith, A L Stratford, T Kogai, L A Tannahill, J C Watkinson, M C Eggo, J A Franklyn, C J Mccabe
Oncogenes 26(30):4344. [CrossRef]

Antitumor Effects of Arsenic Trioxide in Transformed Human Thyroid Cells
Eleonore Fröhlich, Barbara Czarnocka, Peter Brossart, Richard Wahl
18(11):1183. [CrossRef]

Human thyroid tumours, the puzzling lessons from E7 and RET/PTC3 transgenic mice
L Jin, A Burniat, J-E Dumont, F Miot, B Corvilain, B Franc
Br J Cancer 99(11):1874. [CrossRef]

Reciprocal immunohistochemical expression of sodium/iodide symporter and hexokinase I in primary thyroid tumors with synchronous cervical metastasis
Young Ho Jung, J. Hun Hah, Myung-Whun Sung, Kwang Hyun Kim, Soo Youn Cho, Yoon Kyung Jeon
Laryngoscope 119(3):541. [CrossRef]