Transient expression of IL-1β induces acute lung injury and chronic repair leading to pulmonary fibrosis
J. Clin. Invest. Martin Kolb, et al. 107:1529 doi:10.1172/JCI12568 [Go to this article.]

Figure 5
Profibrotic cytokines in BAL fluid. (a) PDGF was increased 2 weeks after injection of AdhIL-1β, 1 week after peak expression of proinflammatory cytokines, and returned to normal values by day 21. (b) TGF-β also showed maximal expression after 2 weeks. By day 21, in contrast to PDGF, concentration of TGF-β in BAL fluid was slightly reduced compared with day 14. Increased TGF-β concentration was measured for the entire course of the experiment up to day 60. (c) The presence of bioactive TGF-β in BAL fluids of AdhIL-1β–treated animals was demonstrated using an established bioassay (MLECs with luciferase reporter gene, controlled by TGF-β–inducible promoter PAI-1; for details see the text). ^AP < 0.04 versus AdDL70 control. ^BP < 0.02 versus AdDL70 control. CP < 0.04 versus PBS.