Apoptosis in podocytes induced by TGF-β and Smad7
J. Clin. Invest. Mario Schiffer, et al. 108:807 doi:10.1172/JCI12367 [Go to this article.]

Figure 3
(a–l) Triple fluorescence labeling using DAPI (a, e, and i), rabbit anti-synaptopodin IgG (b, f, and j), and TUNEL assay (c, g, and k) in renal cortex sections of WT (a–d), 2-week-old TG (e–h), and 5-week-old TG (i–l) mice. TUNEL-positive and DAPI-negative red blood cells (artifacts), yellow arrows; TUNEL-positive podocytes, blue arrows; TUNEL-positive endocapillary/mesangial cells, white arrows. Representative results are shown. (m) Average ± SEM of TUNEL-positive podocytes (black bars) and TUNEL-positive endocapillary/mesangial cells (gray bars) per glomerular section. Data for 2-week-old and 5-week old TG are combined. *TUNEL-positive podocytes in WT vs. 2-week-old TG, P < 0.001. **Podocytes in WT vs. 5-week-old TG, P < 0.05. ⁺Endocapillary/mesangial cells in WT vs. 5-week-old TG, P < 0.01. NS, not significant.