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5-hydroxytryptamine strongly inhibits fluid secretion in guinea pig pancreatic duct cells
Atsushi Suzuki, Satoru Naruse, Motoji Kitagawa, Hiroshi Ishiguro, Toshiyuki Yoshikawa, Shigeru B.H. Ko, Akiko Yamamoto, Hiroyuki Hamada, Tetsuo Hayakawa
Published in Volume 108, Issue 5
J Clin Invest. 2001; 108(5):749–756 doi:10.1172/JCI12312
This article Copyright © 2001, The American Society for Clinical Investigation
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Figure 6

Effects of luminal 5-HT on fluid secretion. The lumen of the cultured ducts was micropunctured and the luminal fluid was replaced by the HEPES-buffered solution with or without 5-HT (1 μM). The ducts were initially superfused with HEPES-buffered solution for 5 minutes. The bath solution was switched to the HCO3–-CO2-buffered solution. After a 6-minute period, 1 nM secretin was added to the bath. (a) The fluid secretory rate in ducts injected with 5-HT (1 μM). Mean ± SEM of four experiments. (b) Spontaneous (HCO3–-CO2–dependent) and secretin-stimulated (1 nM) fluid secretion in the absence (filled bars) and presence (open bars) of luminal 5-HT (1 μM). Mean ± SEM of four experiments.

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