JCI - Modulation of T-cell responses to alloantigens by TR6/DcR3

Modulation of T-cell responses to alloantigens by TR6/DcR3

Jun Zhang, Theodora W. Salcedo, Xiaochun Wan, Stephen Ullrich, Bugen Hu, Theresa Gregorio, Ping Feng, Shijie Qi, Huifang Chen, Yun Hee Cho, Yuling Li, Paul A. Moore, Jiangping Wu

Published in Volume 107, Issue 11

J Clin Invest. 2001; 107(11):1459–1468 doi:10.1172/JCI12159

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Figure 1

TR-6 binds to cell surface LIGHT and competes with TR2 for its binding to LIGHT. (a) Flow cytometry on LIGHT-expressing 293 cells. LIGHT-expressing 293 transfectants or untransfected 293 cells were incubated with TR2-Fc, TR6-Fc, or a control protein TR11-Fc (50 ng/sample), and binding was detected with a PE-conjugated goat anti-human Ig antibody by flow cytometry. Solid lines: LIGHT-expressing 293 cells; shaded areas: untransfected 293 cells. Top row: cells were stained in the absence of competitors; middle and bottom rows: cells were stained in the presence of 10 μg non-Fc TR6 or LIGHT, respectively, as competitors. (b) Flow cytometry on human T cells. Human T cells were activated as described in Methods. Binding of TR2-Fc, TR6-Fc, or TR11-Fc (50 ng/sample) with T cells was detected by flow cytometry using the PE-conjugated goat anti-human Ig antibody. Solid lines: T cells activated with anti-CD3 followed by ionomycin and PMA; shaded area: nonactivated T cells. Top row: cells were stained in the absence competitors; middle and bottom rows: cells were stained in the presence of 10 μg non-FC TR6 or LIGHT, respectively, as competitors.