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Research Article Free access | 10.1172/JCI119656

Myocyte apoptosis during acute myocardial infarction in the mouse localizes to hypoxic regions but occurs independently of p53.

S Bialik, D L Geenen, I E Sasson, R Cheng, J W Horner, S M Evans, E M Lord, C J Koch, and R N Kitsis

Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

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Published September 15, 1997 - More info

Published in Volume 100, Issue 6 on September 15, 1997
J Clin Invest. 1997;100(6):1363–1372. https://doi.org/10.1172/JCI119656.
© 1997 The American Society for Clinical Investigation
Published September 15, 1997 - Version history
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Abstract

Significant numbers of myocytes die by apoptosis during myocardial infarction. The molecular mechanism of this process, however, remains largely unexplored. To facilitate a molecular genetic analysis, we have developed a model of ischemia-induced cardiac myocyte apoptosis in the mouse. Surgical occlusion of the left coronary artery results in apoptosis, as indicated by the presence of nucleosome ladders and in situ DNA strand breaks. Apoptosis occurs mainly in cardiac myocytes, and is shown for the first time to be limited to hypoxic regions during acute infarction. Since hypoxia-induced apoptosis in other cell types is dependent on p53, and p53 is induced by hypoxia in cardiac myocytes, we investigated the necessity of p53 for myocyte apoptosis during myocardial infarction. Myocyte apoptosis occurs as readily, however, in the hearts of mice nullizygous for p53 as in wild-type littermates. These data demonstrate the existence of a p53-independent pathway that mediates myocyte apoptosis during myocardial infarction.

Version history
  • Version 1 (September 15, 1997): No description
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